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Lastly, 1??Ci of 86Rb was extra to every society and incubation was continued at 37?°C for a further 10?min. Reactions have been terminated by washing the cells with ice-chilly a hundred?mM MgCl2 and mobile lysates had been ready for liquid scintillation counting. The 86Rb counts in parallel cultures with ouabain were subtracted from All those wit